Roethlisberger was on the field a week after learning he will not be charged by Georgia prosecutors for alleged sexual assault. The quarterback did not speak to reporters after practice.
The NFL said Monday there is no timetable for a decision on possible punishment, although the Steelers expect the league to act next week.
At a meeting before practice, multiple players said they were told the Steelers now have a zero-tolerance policy regarding misconduct. Wide receiver Santonio Holmes, the MVP of the Steelers' Super Bowl victory in February 2009, was traded to the Jets for a fifth-round draft pick last week following a series of problems.
"We were told early this morning that either you get in line or you'll get kicked out of line — you're going to be traded or you're not going to be here," right tackle Willie Colon said. "If your conduct is going to play a part in you not being a good football player, they're going to get rid of you."
Coach Mike Tomlin denied delivering such a message, but defensive end Aaron Smith confirmed remarks were made to the Steelers "as a group."
"Guys took it different ways, depending on how they interpreted it, but I think … after what we've seen go on, there's not going to be much tolerance for stuff around here," Smith said.
This was a voluntary practice for the Steelers, their first since they concluded a 9-7 season in January. Roethlisberger also participated in off-the-field conditioning work for several days last week.
The quarterback had been accused by a college student of sexual assault in March. Team president Art Rooney II said the Steelers were ready to punish the quarterback, but declined to do so because such action could be contested by the players' union.